One of the Navy's oldest U.S. destroyer squadron organizations. Destroyer Squadron 14 (CDS 14) is the Navy's largest destroyer squadron and is assigned to the Commander Naval Surface Group Two (CNSG-2), whose mission is to conduct maritime operations in support of national interests and unified commanders. COMDESRON 14 is the undersea and surface warfare commander for the USS AMERICA (CV 66) Battle Group (USABATGRU).

As a Regional Support Organization (RSO) and Training and Readiness Squadron, CDS 14 responsibilities are three-fold. As the Immediate Superior in Command (ISIC) of a dozen assigned surface ships homeported in Mayport, Florida and Pascagoula, Mississippi, CDS 14 executes direct oversight of assigned ships during the Maintenance, Unit Level Training and Intermediate Training Phases and discretionary oversight of assigned units while they are deployed in support of scheduled deployments, homeland defense and the Global War on Terrorism.